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PhD defense - Fatima Zahra Alem

19 February 2021
Fatima Zahra Alem
           Fatima Zahra Alem

Date : Friday, February 19th, 2021, 2:00 PM (UTC+1), Salle de conferences, CGFB, Bordeaux.

Title : "Extraction and analysis by liquid chromatography coupled with mass spectrometry of Lawsonia inermis and the study of its anti-melanoma effect".

Summary : Lawsonia inermis L. commonly called Henna belongs to the family Lythraceae. It is known for its traditional importance both as a cosmetic and medicinal agent, especially in the indigenous regions of North Africa, South Asia and Middle East countries. The different parts of this shrub; leaves, stems, seeds and roots have different chemical constituents such as naphthoquinones, polyphenols, flavonoids, tannins, etc, which have various biological activities. The interest of this Mediterranean plant, in addition to its cosmetic use, is its biological activity antimicrobial, anti-inflammatory, anticancer and many others. This research work has been particularly interested in studying its anticancerous effect and more particularly its potential anti-melanoma metastasis given its traditional use often as a topical treatment for irritated skin. This research work allowed, first of all, the development of an experimental plan for the optimization of the global yield of extraction of active molecules from Henna (Lawsonia inermis L.). In the second stage, the identification and quantification of active compounds by high performance liquid chromatography coupled with mass spectrometry.  A geographical mapping of henna crops in southern Morocco, using principal component analysis and based on the nature of the climate of each region was carried out. Subsequently, the effect of plumbagin on metastatic melanoma was studied. The study of this activity was evaluated in-vitro on murine and human cells respectively B16F10 and SKMEL-28. Plumbagin was found to significantly inhibit three important metastatic processes: migration, adhesion and invasion of B16F10 cells. These results were confirmed in the human cell line SK-MEL-28.

Keywords : Lawsonia inermis, naphthoquinones, extraction, HPLC, mass spectrometry, melanoma

Members of the jury : 

Pr. Catia Contado, University of Ferrara, Italy - Reporter
Pr. Amaral Joana Soares, Polytechnic Institute of Bragança, Portugal - Reporter
Pr. Abdelaziz Benjouad, University of Rabat, Morocco - External examiner
Pr. Eddouks Mohammed, University Moulay Ismail - Meknès, Morocco - External examiner
Pr. Rabii Ameziane Hassani, University Mohamed V, Morocco - Internal examiner
Pr. Waffo-Teguo Pierre, University of Bordeaux, France - Internal examiner
Pr. Isoda Hiroko, University of Tsukuba, Japan - Guest member 
Pr. Mayra O.Villareal, University of Tsukuba, Japan - Guest member
Pr. Nadia Dakka, University Mohamed V, Morocco - Guest member
Pr. Zoubida Charrouf, University Mohamed V, Morocco - Guest member
Pr. Boutayna Rhourri-Frih, University of Bordeaux, France - Thesis Director
Pr. Youssef Bakri, University Mohamed V, Morocco - Thesis Director

Information of the PhD : This PhD project is a joint program between Bordeaux University in France and Mohamed V University in Morocco, and a collaboration with Isoda Lab in Tsukuba University, Japan under the supervision of Pr Isoda Hiroko. The doctoral project has been financially supported by the excellence doctoral scholarship EIFFEL from French government and Japan Student Services Organization (JASSO) from Japan.