Proteomics Platform continuously expanded its range of service focusing on the separation, identification and characterization of proteins, differential protein quantification or finding protein partners.
More specifically, in the field of protein separation, the Plateforme Proteome offers electrophoretic techniques (SDS-PAGE, BN-PAGE 16-BAC-PAGE, IEF) and two-dimensional (BN / SDS-PAGE, 16 - BAC / SDS-PAGE, IEF / SDS-PAGE). The gels can be stained with a variety of dye (Coomassie Blue, Colloidal Blue, Silver (MS Compatible), Pro Q Diamond, Ruthenium II, Sypro, Deep Purple). The gel images can then be processed (Image Analysis / biostatistics) to determine the spots whose intensity is modulated significantly different between conditions (differential expression proteomics).
The gels can also be transferred by Western.
Alternatively, proteins can be separated by isoelectric focusing in liquid stream either through the system Rotofor or through the system OffGel.
With regard to identification, protein spots can be identified by MALDI-TOF/TOF and LC-MS/MS on ion trap.
The more complex mixtures are analyzed by LC-MS/MS on a high-resolution Orbitrap type. The length of the gradient is adapted to the complexity of the sample.
In the field of quantitative proteomics, the Plateform Proteome offers the possibility to analyze your samples via the Label-Free technology. It allows to compare an "unlimited" number of samples without proteins / peptides labeling step. The choice of analysis strategy is guided by the sample volume, the required quantities and the upstream experimental design.
The mass measurement of intact proteins can be in direct analysis (if pure samples) or by LC-MS.
In the absence of adequate data bank, the identification of proteins by de novo sequencing is possible.
Finally, the Plateform Proteome proposes various strategies for the characterization of post-translational modifications (i.e. phosphorylation, ubiquitinylation, glycosylation).
Before submitting samples, it is advisable to contact the platform to establish an analysis strategy and discuss on sample preparation conditions. The services are supplied either as a collaboration or as a service.
- In the case of collaboration, the laboratory carrying the scientific project assumes operating costs. In this case, agents involved in service activities will be cited as co-authors of scientific publications.
- In the case of a service, the carrier of laboratory science project assumes not only operating costs but also the cost of expertise. In this case, the laboratory project sponsor retains ownership of the results.
In any case, please contact Marc Bonneu.
An invoice will be established on request. For example, the cost of an analysis by LC-MS / MS Q-Exactive with a gradient of 2 hours is 85.4 € HT in collaborative modeand € 298.8 HT in service mode. This last price is decreasing according to the number of samples processed simultaneously.