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Proteome Intruments

Mass spectrometry :

  • AP-SMALDI5 AF Q-Exactive
    AP-MALDI ion source (TransMIT) + Q-Exactive (Thermo Scientific).
    Operational since 2019 (Source) / Operational since 2013 (Mass spectrometer)
     
  • LTQ Orbitrap XL
    U3000 (Dionex)/Automate HDX (LEAP) + LTQ Orbitrap XL ETD (Thermo Scientific).
    Operational since 2009
     
  • Q-TRAP 5500
    Nexera XR (Shimadzu) + QTRAP 5500 System (AB Sciex).
    Operational since 2009
  • MALDI-TOF Mass Spectrometer
    Ultraflex III TOF-TOF (Bruker Daltonics) + High Mass Detector HM2 (CovalX)
    Operational since 2008
  • Q-TOF Premier
    U3000-RSLC (Thermo Scientific) + Q-TOF premier (Waters Micromass).
    Operational since 2006
  • Q-TOF Micro
    CapLC + Q-TOF micro (Waters Micromass).
    Operational since 2005

 

Bioinformatics :

  • Bioinformatics platforms : Proteome Discoverer 2.4 (Thermo Scientific).
  • Identification softwares : Sequest HT (Thermo Scientific), MS Amanda (Thermo Scientific), Mascot 2.5 (Matrix Science), Peaks (Bioinformatics Solutions Inc), Prosight PD (Thermo Scientific).
  • Quantification softwares : Progenesis QI (Nonlinear Dynamics), Proteome Discoverer 2.4 (Thermo Scientific).
  • Caracterization softwares : Byonic 2.0 (Protein Metrics).
  • Imaging softwares : FlexImaging 4.0 (Bruker Daltonics), SCiLS.

 

Servers and storage :

  • Data Archiving: 80TB RAID 5.
  • Computing servers.
  • Hosting in server room under environmental monitoring.

 

Protein biochemistry : 

  • Monodimensional electrophoresis gels, colorations : commassie bleu, colloidal blue, silver, sypro ruby.
  • IsoElectricFocusing systems : OffGel (Agilent), Rotofor (Biorad)
  • Two-dimensional electrophoresis gels 24 cm :
    • 1th dimension   IPG Phor (Amersham Biosciences), Protean IEF Cell (BioRad)
    • 2nd dimension  Ettan Dalt Six ou twelve (Amersham Biosciences), Protean II Multicell (BioRad)
  • Two-dimensional electrophoresis gels 6 cm :
    • 1th dimension   IPG Runner (Invitrogen)
    • 2nd dimension  Sure Lock (Invitrogen)
  • Image analysis : Fluorescence scanner Pharos FX™ Plus with external laser molecular imager FX (BioRad), ImageMaster™ 2D Platinum v5.0 (Amersham Biosciences), Quantity One 4.6.2 (BioRad), PDQuest 8.0.1 (BioRad), Progenesis SameSpots V2.0 (Non Linear Dynamics)
  • Spot cutter : EXQuest (BioRad)
  • Liquid and in gel digestion of proteins by several proteases.